Two accomplished genomics scientists, Dr. Sasha Levy and Professor Gavin Sherlock, and Dave Craford, an experienced life sciences CEO, co-founded BacStitch DNA to develop and commercialize a transformative platform technology that enables highly efficient DNA engineering and will advance the development of Synthetic Biology solutions to problems in medicine, manufacturing and agriculture.
Current workflows that engineer DNA that codes for multiple genes and pathways are complex, difficult to automate, and expensive. BacStitch will replace these with simple, easy to automate, cost effective workflows where bacteria, instead of people, do most of the work.
The technology involves programming bacteria to stitch "bac-stitch" and move DNA. In addition to efficiently assembling long DNA constructs (currently have feasibility data for >25kb) the platform can manipulate complex DNA with high GC content and repeats. It can also introduce directed variation in multiple user-definable regions within the constructs and the component parts of the constructs can easily be rearranged to create well-balanced combinatorial libraries.
Here's a recent bioRxiv preprint that describes use of the platform for plasmid sequencing and library demultiplexing.
BacStitch is building a team of outstanding scientists and resides in Johnson and Johnson's JLABS life science incubator in South San Francisco.
329 Oyster Pt. Blvd. 3rd Floor South San Francisco, CA 94080