BacStitch™ Programs Bacteria to Engineer DNA

BacStitch™ Programs Bacteria to Engineer DNA

A diagram of the process of dna synthesis.

In vitro

  • Many Enzymatic, Purification and Screening Steps
  • Considerable Technician Hands on Time
  • Difficult to Automate
  • Unpredictable for Large Assemblies
  • Costly
A drawing of a toothbrush with two different colors.

In vivo

  • Simple, Highly Scalable Mating of Bacteria
  • Minimal Technician Hands-On Time
  • Easy to Automate in Arrays
  • Predictable Stepwise Process
  • Step Change Reduction in Cost

Posters and Publications

Scalable DNA engineering by mating bacteria in arrays and pools, SynBioBeta 2024

Arrayed in vivo barcoding for multiplexed sequence verification of plasmid DNA and demultiplexing of pooled libraries, Nucleic Acids Research, June 2024

High-throughput DNA engineering by mating bacteria, bioRxiv, September 2024